ABSTRACT
A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever (DF) by evaluating biochemical and oxidative status of patients. ELISA IgM antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) showed significant difference. Significant increases in serum alanine amino transferase (ALT) (P=0.000), aspartate amino transferase (AST) (P=0.000), alkaline phosphatase (ALP) (P=0.000), malondialdehyde (MDA) along with significant decreases in total protein (TP) (P=0.000), reduced glutathione (GSH) (P=0.000), superoxide dismutase (SOD), catalase (CAT) (P=0.000), and sialic acid contents (P=0.016) were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants (r=0.656, P=0.016). In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Antioxidants , Metabolism , Biomarkers , Blood , China , Dengue , Classification , Diagnosis , Metabolism , Diagnosis, Differential , Immunoglobulin M , MetabolismABSTRACT
The aim of the present study was to investigate the changes in biochemical parameters including lipid profile, liver and kidney profile as well as oxidative stress profile, particularly in patients suffering from alcoholic liver disease [ALD]. Fifty chronic alcoholics admitted for treatment to the in-patient wards at Jinnah Hospital, Lahore-Pakistan. Chronic alcoholics with alcohol abuse for more than four to five years, and with or without clinical complications, were included. Apparently twenty healthy individuals served as control. 2012-2013. Various circulating biochemical biomarkers including renal profile, hepatic and lipid profile were evaluated. Moreover, stress markers [MDA. SOD, GSH and catalase] were also investigated. A very strong direct and indirect correlation of ALP was found with TB, MDA and GSH [r=.950**, r=.929** and r=-.967** respectively, P<0.01]. MDA was observed having very strong indirect correlation with GSH and catalase [r=-.909** and r-.777** respectively, P<0.01]. All parameters in combinations may be useful indicator or may be good and reliable biochemical markers for identification and determination of severity of alcoholic liver diseases [ALD]. The damaging of hepatocytes due to the consumption of alcohol disturbs almost all types of biochemical coordination in the biological system
Subject(s)
Humans , Oxidative Stress , Lipids , Liver Function Tests , Kidney Function TestsABSTRACT
Objective: The Urticaria caused by NSAIDs wasstudied for their antioxidant enzyme activity andmalondialdehyde [MDA] concentration
Methodology: Patients were divided into twogroups; group one act as a control and the remaininggroup was urticaria patients. The levels of theThiobarbituric acid reactive substances [TBARS, asa marker of lipid peroxidation], superoxidedismutase [SOD], glutathione [GSH] and catalase[CAT] in the serum were estimated. The enzymaticactivity was estimated and compared with differentcategories of NSAIDs
Results: In present studyCAT, SOD and lipid peroxidation product [MDA]were statistically different from that of healthycontrol and showed significant changes [p< 0.05]while GSH showed a non-significant [p>0.05]value
Conclusion: It is concluded thatantioxidative system of the body is accelerated andshows variations in the patient of Urticaria.Moreover, present study shows the co-ordinatedenzymatic mechanism and the interrelationshipbetween enzymatic activities in allergy